Disease: Leprosy (Hanse's disease) Enzyme class: 2; Transferases *Target (protein/gene name):Dihydroteroate synthase *NCBI Gene # or RefSeq#: -- *Protein ID (NP or XP #) or Wolbachia#: -- *Organism (including strain):Mycobacterium Leprae *Etiologic Risk Group:
Group 3 http://www.bacterio.net/-hazard.html#group3
*Disease Information:
Leprosy, which is also known as Hansen's disease is an infectious disease by Mycobacterium leprae. They can affect human in various ways by making human lose the ability to sense touch or pain. When injuries happen, people will not know if they are in danger because their first defense line is gone. They affect nerves, skin, eyes, and nasal mucosa. The affected area becomes either light or dark and become dry. They can also be inflamed. Without cure, they can lead to a paralysis of limps. They are contagious, but there is an easy cure to this disease now. However, in third world countries, leprosy is still around affecting many people. https://www.cdc.gov/leprosy/
Link to TDR Targets page: n/a Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.) Essentiality of this protein: --
Expression Information (has it been expressed in bacterial cells): -- Purification Method: -- Image of protein (PyMol with features delineated and shown separately):
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
*length of your protein in Amino Acids
284 Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website
29.448 kDa Molar Extinction coefficient of your protein at 280 nm wavelength:
<span style="color: #626262;">Ext. coefficient 31970
Abs 0.1% (=1 g/l) 1.086, assuming all pairs of Cys residues form cystines
Ext. coefficient 31970
Abs 0.1% (=1 g/l) 1.086, assuming all Cys residues are reduced</span>
TMpred graph Image (http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it. *CDS Gene Sequence (paste as text only): atgagcctggcgccggtgcaggtgattggcgtgctgaacgtgaccgataacagctttagc gatggcggccgctatctggatccggatgatgcggtgcagcatggcctggcgatggtggcg gaaggcgcggcgattgtggatgtgggcggcgaaagcacccgcccgggcgcgattcgcacc gatccgcgcgtggaactgagccgcattgtgccggtggtgaaagaactggcggcgcagggc attaccgtgagcattgataccacccgcgcggatgtggcgcgcgcggcgctgcagagcggc gcgcgcattgtgaacgatgtgagcggcggccgcgcggatccggcgatggcgccgctggtg gcggaagcgggcgtggcgtgggtgctgatgcattggcgcctgatgagcgcggaacgcccg tatgaagcgccgaactatcgcgatgtggtggcggaagtgcgcgcggatctgctggcgggc gtggatcaggcggtggcggcgggcgtggatccgggcagcctggtgattgatccgggcctg ggctttgcgaaaaccggccagcataactgggcgctgctgaacgcgctgccggaactggtg gcgaccggcgtgccgattctgctgggcgcgagccgcaaacgctttctgggccgcctgctg gcgggcgcggatggcgcggtgcgcccgccggatggccgcgaaaccgcgaccgcggtgatt agcgcgctggcggcgctgcatggcgcgtggggcgtgcgcgtgcatgatgtgcgcgcgagc gtggatgcgctgaaagtggtgggcgcgtggctgcatgcgggcccgcagattgaaaaagtg cgctgcgatggc
*GC% Content for gene: 69.014085 *CDS Gene Sequence (codon optimized): n/a *GC% Content for gene (codon optimized): n/a
Do Not Need this info for Spring (but still copy these lines to your Target page for now) Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers): (link to DNA Works output text file - that should be saved in your Google Docs folder after you did the primer design protocol) -- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
Primer design results for 'tail' primers (this is just 2 sequences): **
Enzyme class: 2; Transferases
*Target (protein/gene name): Dihydroteroate synthase
*NCBI Gene # or RefSeq#: --
*Protein ID (NP or XP #) or Wolbachia#: --
*Organism (including strain): Mycobacterium Leprae
*Etiologic Risk Group:
Group 3
http://www.bacterio.net/-hazard.html#group3
*Disease Information:
Leprosy, which is also known as Hansen's disease is an infectious disease by Mycobacterium leprae. They can affect human in various ways by making human lose the ability to sense touch or pain. When injuries happen, people will not know if they are in danger because their first defense line is gone. They affect nerves, skin, eyes, and nasal mucosa. The affected area becomes either light or dark and become dry. They can also be inflamed. Without cure, they can lead to a paralysis of limps. They are contagious, but there is an easy cure to this disease now. However, in third world countries, leprosy is still around affecting many people.
https://www.cdc.gov/leprosy/
Link to TDR Targets page: n/a
Link to Gene Database page (NCBI, EuPath databases -e.g. TryTryp, PlasmoDB, etc - or PATRIC, etc.)
Essentiality of this protein: --
Complex of proteins?: --
Druggable Target:
https://www.ncbi.nlm.nih.gov/pubmed/10329458
*EC#: 2.5.1.15
Link to BRENDA EC# page:
http://www.brenda-enzymes.org/enzyme.php?ecno=2.5.1.15&UniProtAcc=P0C0X1&OrganismID=3504
Enzyme Assay information:
https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/dihydropteroate-synthase
List cost and quantity of substrate reagents, supplier, and catalog #
Structure
-- PDB #l: Q9R2U9
Current Inhibitors:
http://www.brenda-enzymes.org/enzyme.php?ecno=2.5.1.15&UniProtAcc=P0C0X1&OrganismID=3504
Expression Information (has it been expressed in bacterial cells): --
Purification Method: --
Image of protein (PyMol with features delineated and shown separately):
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
<span style="color: #222222; font-family: monospace,monospace; font-size: 14.039999961853027px;"> 10 20 30 40 50 MSLAPVQVIG VLNVTDNSFS DGGRYLDPDD AVQHGLAMVA EGAAIVDVGG 60 70 80 90 100 ESTRPGAIRT DPRVELSRIV PVVKELAAQG ITVSIDTTRA DVARAALQSG 110 120 130 140 150 ARIVNDVSGG RADPAMAPLV AEAGVAWVLM HWRLMSAERP YEAPNYRDVV 160 170 180 190 200 AEVRADLLAG VDQAVAAGVD PGSLVIDPGL GFAKTGQHNW ALLNALPELV 210 220 230 240 250 ATGVPILLGA SRKRFLGRLL AGADGAVRPP DGRETATAVI SALAALHGAW 260 270 280 GVRVHDVRAS VDALKVVGAW LHAGPQIEKV RCDG </span>*length of your protein in Amino Acids284
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website
29.448 kDa
Molar Extinction coefficient of your protein at 280 nm wavelength:
TMpred graph Image (http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
*CDS Gene Sequence (paste as text only):
atgagcctggcgccggtgcaggtgattggcgtgctgaacgtgaccgataacagctttagc gatggcggccgctatctggatccggatgatgcggtgcagcatggcctggcgatggtggcg gaaggcgcggcgattgtggatgtgggcggcgaaagcacccgcccgggcgcgattcgcacc gatccgcgcgtggaactgagccgcattgtgccggtggtgaaagaactggcggcgcagggc attaccgtgagcattgataccacccgcgcggatgtggcgcgcgcggcgctgcagagcggc gcgcgcattgtgaacgatgtgagcggcggccgcgcggatccggcgatggcgccgctggtg gcggaagcgggcgtggcgtgggtgctgatgcattggcgcctgatgagcgcggaacgcccg tatgaagcgccgaactatcgcgatgtggtggcggaagtgcgcgcggatctgctggcgggc gtggatcaggcggtggcggcgggcgtggatccgggcagcctggtgattgatccgggcctg ggctttgcgaaaaccggccagcataactgggcgctgctgaacgcgctgccggaactggtg gcgaccggcgtgccgattctgctgggcgcgagccgcaaacgctttctgggccgcctgctg gcgggcgcggatggcgcggtgcgcccgccggatggccgcgaaaccgcgaccgcggtgatt agcgcgctggcggcgctgcatggcgcgtggggcgtgcgcgtgcatgatgtgcgcgcgagc gtggatgcgctgaaagtggtgggcgcgtggctgcatgcgggcccgcagattgaaaaagtg cgctgcgatggc
*GC% Content for gene: 69.014085
*CDS Gene Sequence (codon optimized): n/a
*GC% Content for gene (codon optimized): n/a
Do Not Need this info for Spring (but still copy these lines to your Target page for now)
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
(link to DNA Works output text file - that should be saved in your Google Docs folder after you did the primer design protocol)
-- Ask a mentor, Dr. B, or a fellow researcher -how to link a GDocs file if you are not sure how to.
Primer design results for 'tail' primers (this is just 2 sequences):
**